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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Cell Cycle Reactivation, at the Start of Neurodegeneration, Induced by Forskolin and Aniline in Differentiated Neuroblastoma Cells
doi: 10.3390/ijms241814373
Figure Lengend Snippet: Dual color immunolocalization of tau epitopes in SH-SY5Y cells. ( A – C ) Replicative SH-SY5Y cells with the visualization of Tau-1, Tau-5, and AT8 epitopes, respectively. ( D – F ) Differentiated SH-SY5Y cells with the visualization of Tau-1, Tau-5, and AT8 epitopes, respectively. Tau-1, Tau-5, and AT8 were revealed by FITC-conjugated antibodies (green signals). Ki-67 (replication marker) and UBTF (nucleolar marker) were detected by TRITC-conjugated antibodies (red signals). DAPI (blue signals) was used to stain cell nuclei. White arrow in ( E ) indicates a cell nucleus with the presence of the Ki-67 marker. White arrow in ( F ) indicates the co-localization of AT8 and UBTF in a nucleus. Magnification is the same for all the images, with a unique scale bar shown in ( F ): 10 μm. The images were captured by confocal laser scanning microscope at 630× magnification. Software to analyze signal co-localization was ZEN-2010 (see ).
Article Snippet: The immunodetection was analyzed using a confocal laser scanning microscopy (CLSM) (LSM700, Zeiss, Oberkochen, Baden-Württemberg, Germany) and images were captured at 400× and/or 630× magnification using the
Techniques: Marker, Staining, Laser-Scanning Microscopy, Software
Journal: International Journal of Molecular Sciences
Article Title: Cell Cycle Reactivation, at the Start of Neurodegeneration, Induced by Forskolin and Aniline in Differentiated Neuroblastoma Cells
doi: 10.3390/ijms241814373
Figure Lengend Snippet: Example of co-localization of IIF signals in the cell nucleus. ( A ) serial section along the z-axis of a cell showing detection of tau (green signals) and ki-67 (red signals). Each image (from 1 to 15) corresponds to a serial section of 0.3 µm thickness. ( B ) Orthogonal view of the same cell shown in ( A ). In the upper part is presented a view of cell section along the green horizontal line. In the right part is presented a view of the cell section along the red vertical line. ( C ) The same cell is shown in ( A ) with the fluorescence intensity (graph on the left) of the tau (green), Ki-67 (red), and DAPI (blue) along the red line indicated by the number 1. The images show the localization in the same nuclear compartment of the analyzed epitopes. Scale bars (5 µm) were indicated for each panel ( A – C ). Images were produced with the ZEN-2010 software.
Article Snippet: The immunodetection was analyzed using a confocal laser scanning microscopy (CLSM) (LSM700, Zeiss, Oberkochen, Baden-Württemberg, Germany) and images were captured at 400× and/or 630× magnification using the
Techniques: Fluorescence, Produced, Software